Create composite rgb image from 2 ImagePlus objects

Posted by ampersand on
URL: http://imagej.273.s1.nabble.com/Create-composite-rgb-image-from-2-ImagePlus-objects-tp3695334.html

Hi all,

I am writing an imagej plugin that does the following:

1. Loads an image that has been acquired with a 'dual-view' , i.e. the
image is grayscale and split down the middle,where one half represents
one color and the other corresponds to a different color.
2. Using ROIs, splits the image into two separate ImagePlus objects of
equal size (both equal to 1/2 the size of the original)
3. Combines the two resultant images into an RGB or composite image
with the two colors represented by separate channels


The problem is with this last step. I can use the RGBMerge plugin from
the menu, or put the two images into a stack and make an RGB from
that, but all this is done from the menus. How can I implement this
programmatically? Again, the end result should be a composite image,
w/ channels whose balance,level,contrast,brightness,etc can be
adjusted separately.


Any help is appreciated!


Regards,
Alex