Posted by
Sami Badawi-2 on
Aug 15, 2008; 9:16am
URL: http://imagej.273.s1.nabble.com/Help-needed-in-doing-image-analysis-tp3695355p3695356.html
Hi Kashif,
Here is a suggestion:
1: Since your petri dish is always round, you could do a circle
selection inside its edge, in order to avoid the shadows and
highlights on the edge that could be counted as particles.
2: Fill everything outside your circle with the background color
(making it all disappear).
4: Write down the radius or diameter of your circle since this will
give you the area of your dish.
5: Run a particle analyzer on the image.
6: Export the result to an Excel spreadsheet
7: Add circle radius to each spreadsheet for calculating density.
There is a mature particle analyzer coming with ImageJ, it should
probably do what you need based on your images.
There is also a particle analyzer with different properties coming
with the ShapeLogic plugin.
-Sami Badawi
http://www.shapelogic.orgOn 8/11/08, Kashif Zeeshan <
[hidden email]> wrote:
> Hello Everybody,
>
> I need your help please. I have a fungus which produce different kinds/forms
> of aggregates (pellets, clumps etc) in different media. I am giving a name
> "aggregates" to all the forms and my objective is to measure the size/area,
> diameter, density etc of all these aggregates. I am in search of a method
> which I can use for all of these different forms to do analysis. I mean that
> a same analysis method which can be used for all images. I am sending you
> the link where I put some of the pictures just to give you an idea how these
> aggregates can be. Can you help me in this regard please? If you can guide
> me step by step, it will be really very much helpful and I will be very
> thankful to all of you. Here is the link.
>
>
>
>
> <
http://s305.photobucket.com/albums/nn227/ablaze212/Images%20for%20Analysis/>>
>
http://s305.photobucket.com/albums/nn227/ablaze212/Images%20for%20Analysis/>
>
>
> Cordially.
>
>
>
> Kashif ZEESHAN
>
> (00 33 6 74 90 18 10)
>
> Doctorant, Biopesticide Group,
>
> Laboratoire Universitaire de
>
> Biodiversité et d'Ecologie
>
> Microbienne (LUBEM),
>
> 6 Rue de l'Université,
>
> 29334, Quimper Cedex,
>
> France
>
>
>