3D object colocalization

Posted by Adelaine Leung on Jul 16, 2008; 8:46pm
URL: http://imagej.273.s1.nabble.com/how-to-measure-tp3695568p3695571.html

Hello all,

I'm new to ImageJ and hope that someone can give me some advice on  
what I'd like to do.  I'm trying to highlight and count  
colocalization of two types of antibody labeling.  Both antibodies  
label many nuclei and I'm trying to identify the few that have  
colocalization.  What I want to do is the following:

1.  Identify 3D objects of the labeled nuclei for each antibody.  
(Channel 1 and 2)
2.  Highlight all the colocalized pixels using the Colocalization  
Highlighter plugin.  (Channel 3)
3.  Creat 3D objects for the colocalized voxels. (Channel 3)
4.  Compare the 3D objects in all three channels, filter out the  
objects that don't have the same (or within a certain threshold)  
volume in all three channels, then count what's left.

I figured out how to do the first three tasks.  I'm not sure what's  
the best way to deal with the fourth one.

Thanks very much for your help!

Adelaine


Adelaine Leung
Kravitz Lab
Department of Neurobiology
Harvard Medical School
220 Longwood Avenue
Boston, MA 02115, USA
Phone: [phone]
Fax: [phone]