Two color detection + segmentation

Posted by Marko Usaj on Jul 09, 2008; 12:18pm
URL: http://imagej.273.s1.nabble.com/Grains-analysis-in-metals-tp3695638p3695640.html

Hello everybody!

I have following problem:

I have fluorescence images 1 with objects (intensity) 1 and fluorescence images 2 with objects (intensity) 2. But some of object are the same (fused) I want to find how many (percent) object are dual color.

Ok, one option is segmentation and then comparison/color align and then counting.

But i hear that similar procedure is to just compare pixel intensity, and perform percent calculation using number of pixels instead of object (dual color/all). Is there any plug in for do that?

Second questions is about uneven illumination. Can anyone suggest good plugin who deal with that.

Third questions is fluorescence thresholding/segment fluorescence nuclei of cells. Which is good algorithm/plugin for that?

Regardss

Marko