Iterative Deconvolution

Posted by Milad Alemzadeh on Jul 01, 2008; 7:49pm
URL: http://imagej.273.s1.nabble.com/Iterative-Deconvolution-tp3695700.html

Dear all,

I am using Bob Dougherty's Iterative Deconvolution plugin. I was wondering
when using experimental PSF images, what size would be appropriate for a
PSF? What I understand is the plugin only considers the PSF width in pixel
and not the physical width (DPI). Therefore, if I take an image of a PSF
with higher resolution, the size of PSF in pixel increases but the final
deconvolved image will be degraded.

What pixel size should I use to record my beads as PSF?

Thanks,
Milad