Re: Iterative Deconvolution

Posted by Robert Dougherty on Jul 02, 2008; 3:23am
URL: http://imagej.273.s1.nabble.com/Iterative-Deconvolution-tp3695700p3695701.html

Milad,

I'm not sure I understand the question, but the general idea is to make the
PSF and the images you plan to deconvolve have the same number of microns
per pixel.

Bob

Robert P. Dougherty, Ph.D.
President, OptiNav, Inc.
Phone [phone]
Fax [phone]
www.optinav.com
 

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Milad Alemzadeh
> Sent: Tuesday, July 01, 2008 12:50 PM
> To: [hidden email]
> Subject: Iterative Deconvolution
>
> Dear all,
>
> I am using Bob Dougherty's Iterative Deconvolution plugin. I was wondering
> when using experimental PSF images, what size would be appropriate for a
> PSF? What I understand is the plugin only considers the PSF width in pixel
> and not the physical width (DPI). Therefore, if I take an image of a PSF
> with higher resolution, the size of PSF in pixel increases but the final
> deconvolved image will be degraded.
>
> What pixel size should I use to record my beads as PSF?
>
> Thanks,
> Milad