Re: Western Blot

Posted by diah taman on Jun 24, 2008; 8:37am
URL: http://imagej.273.s1.nabble.com/Western-Blot-tp3695711p3695713.html

Dear Veronica,
you can use it. Its very easy to do, like below:
1.open your file (western blott do you have),
2. klik image/color/RGB split
3. chose one of figure
4. use rectangular to select "band" do you need for analyze
5. klik ctrl+1
6. klik analyze/plot profile
you find result in grey scale. If you want to change scale to "OD". you should make "optical density caliberation.
open http://rsb.info.nih.gov/ij/docs/examples/calibration/index.html
 
Have a nice day,
 
Best wishes,
 
Al Munawir, MD
Toxicology Molecular Lab.
Gyeong Sang National University
900 Gajwa-Dong, Jinju 660-701
South of Korea


--- On Tue, 6/24/08, Veronica Dy <[hidden email]> wrote:

From: Veronica Dy <[hidden email]>
Subject: Western Blot
To: [hidden email]
Date: Tuesday, June 24, 2008, 12:54 PM

Dear ImageJ users,

I have a quick question.

Am I able to use ImageJ to analyse the bands I have from Western Blot
analyses?  If so, what is the best plugin to use?

Thank you very much for your time.

Regards,
Veronica.