Re: Color Deconvolution and the subsequent image merge

Posted by jchanson on Jun 24, 2008; 6:28pm
URL: http://imagej.273.s1.nabble.com/Color-Deconvolution-and-the-subsequent-image-merge-tp3695746p3695747.html

Przemko -
        Since the white has a value of "255", these images need to be
inverted (to make the background "0")  before adding them together.  Try -

- (running Color Deconvolution)
- (enhancing the colors as you see fit)
- convert images to RGB (Image | Type | RGB Color)
- invert both images (Ctrl-Shift-i),
- add them together (Process | Image Calculator)
- and then invert the final image

Jeff
Senior Imaging Analyst
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Subject
Color Deconvolution and the subsequent image merge






Hi!
The title says it all. I have DAB (brown) stained tissue sections,
counterstained with hematoxylin (a result of immunohistochemistry).
Since my brown staining is sometimes weak, I would like to enhance it. I
used Color Deconvolution plugin to separate the colors (GREAT PLUG-IN!)
and enhance the brown color. The problem however starts here. I would
like to put my images back, but to no avail. Stacks don't work, RGB
MERGE changes colors, Image calculator changes colors, Color plugin
changes colors. So, How could I separate the two color spaces, enhance
one of the colors (that far I can do it) and then merge the images back
to get the same blue (from hematoxylin) and enhanced brown (from DAB). I
looked in various tutorials etc but cannot find anything.
TIA
Przemko

--
Przemko Tylzanowski Ph.D.
LSD & Joints
O & N1, box 813- dark green
University of Leuven
Herestraat 49
3000 Leuven, Belgium

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