Posted by
Gabriel Landini on
Jun 26, 2008; 11:56am
URL: http://imagej.273.s1.nabble.com/Color-Deconvolution-and-the-subsequent-image-merge-tp3695746p3695748.html
On Tuesday 24 June 2008 19:28:13 Jeffrey C Hanson wrote:
> - (running Color Deconvolution)
> - (enhancing the colors as you see fit)
Since I wrote that plugin let me just add some further comment, for the
record.
Before anybody thinks that this is a good idea, enhancing weak immunostains
selectively (i.e. separating dyes, adjust one of them and re-merge them to
then recreate an enhanced image), is likely to make unaware observers believe
that antibody avidity is higher than it really is, and/or that the antigen
presence is more prominent that it is.
Of course I am not saying that the original post was suggesting to do any of
this, but I think that, peer reviewed journals are likely see this "practice"
as misrepresentation of the data.
There are a few articles about this type of problem which are an interesting
read:
http://www.jcb.org/cgi/content/full/166/1/11I would strongly advice against doping that. I would just leave weak staining
to remain weak. Otherwise, there is no point in following staining protocols
with controlled times, dilutions and temperatures.
There are enough problems already with the arbitrary meaning of immunostain
intensity.
Regards.
Gabriel