Re: Fluorescence quantification

Posted by John Oreopoulos on Jun 19, 2008; 4:33pm
URL: http://imagej.273.s1.nabble.com/Fluorescence-quantification-tp3695829p3695830.html

Hi Sofia,

If you draw a rectangular region of interest with the rectangular  
selection tool in ImageJ and then click Analyze->Measure, you'll get  
a results table that lists the average pixel intensity ("green  
signal") over the area you have drawn. On a more technical/
experimental note, I would caution you against trying to make a  
correlation between how close to death a cell is (via apoptosis) and  
the amount of greet signal. There are many other factors in a live  
cell fluorescence imaging experiment that can lead to changes in the  
amount of signal. For instance, See Jim Pawley's "39 steps" article  
in Biotechniques.

John Oreopoulos


On 19-Jun-08, at 12:00 PM, Sofia Domingues wrote:

> Hi there,
> I am working with GFP astrocytes and I would like to quantify the  
> loss of
> fluorescence in the process of apoptosis, as when they die they  
> detach from
> the wells and become very small round green cells.
> Does anyone know wether there is a plug-in on Image J to quantify  
> the "green
> signal" per area?
> Thanks
> Sofia