Posted by
Kashif Zeeshan on
URL: http://imagej.273.s1.nabble.com/Shadow-problem-tp3695874p3695876.html
Hi,
Thanks for your nice suggestions. Actually, I am totally new to ImageJ and
up to now it seems me very difficult to use this. Can you kindly tell me
from where to start? The images I am using are colored imaged taken through
an ordinary camera (5M pixels) in the neon light. The liquid medium
containing aggregates was being poured in a large Petri dish and then the
photos were being taken from the above. To avoid the reflection of the light
and camera, I placed the Petri dishes in a corner of a room but due to this
now I have the shadows in the images. With the help of the replies of this
group experts, I was able to do some analysis. But ImageJ count these
shadows either as a part of the aggregates or separate aggregates. I read
the paper sent in the previous email. Thanks again for this help. But where
I will find this option of HSV color-space? I am sorry that most of the
times these terms given by the experts are difficult also and I am not able
to understand all. Can you kindly help me to sort out the problem? Where I
can find this option or how can I do this please?
Cordially.
Kashif ZEESHAN
(00 33 6 74 90 18 10)
Doctorant, Biopesticide Group,
Laboratoire Universitaire de
Biodiversité et d'Ecologie
Microbienne (LUBEM),
6 Rue de l'Université,
29334, Quimper Cedex,
France
-----Message d'origine-----
De : ImageJ Interest Group [mailto:
[hidden email]] De la part de David
Randell
Envoyé : jeudi 12 juin 2008 11:22
À :
[hidden email]
Objet : Re: Shadow problem
On Thursday 12 June 2008 09:15:32 Kashif Zeeshan wrote:
> I have the problem of shadows in the image. When I try to count the
> particles, it considers either the shadows of the particles as their part
> or another particle and thus falsifies the results. Can you kindly help me
> in this regard? How can I overcome this problem? Is there any plugin or
> easy method to remove the shadows from the image?
Are the images grayscale or colour? If the latter and the objects are
reasonably homogeneous in colour you might try identifying and filtering out
the shadows by converting the image into a HSV colour-space, and
manipulating
the H and V channels. See this paper for an idea how to approach this
particular problem:
http://ir.lib.u-ryukyu.ac.jp/bitstream/123456789/1489/1/No66p71.pdfBest regards,
Dave Randell