Flat Field Correction for fluorescence

Posted by Pang, Zhengyu (GE Global Research) on Jun 04, 2008; 3:10pm
URL: http://imagej.273.s1.nabble.com/Flat-Field-Correction-for-fluorescence-tp3696040.html

Dear all,

I am doing flat field correction for fluorescence image by taking a
image of a plastic colored bar (Image1). The orignal image for my target
is Image2. I applied image calculator function and used divide. The
resulting image is a 32-bit image (Image3). I then use
Process/Math/multiply. Ideally, I want image 3 has the same average
intensity as Image2 and a 16-bit image (technical my raw image is 12bit,
and the pixel value is from 0-4095). How could I do it?

In addition, I can measure the average intensity for Image2 using
Run("measure"), but how could I get that value from the Results window
and use that value in the macro?

Thanks!

Zhengyu
> g Global Research Center
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> ______________________________________________________________________
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>           Zhengyu Pang, Ph.D.
                        Bioanalytics and Protein Science Laboratory
                        Biosciences Global Technology Organization
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> Niskayuna, NY12309
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