subtraction of images

Posted by Oliver Bannach on Jun 04, 2008; 9:22am
URL: http://imagej.273.s1.nabble.com/subtraction-of-images-tp3696059.html

Dear imagej experts

What I like to do is basically a subtraction of images (imageA minus
imageB). But each pixel yielding a signal in imageA must be set to zero,
if it also appears in imageB, regardless of signal intensities. In other
words: Any colocalized pixel must be black in image A. The conventional
subtract process (Process->Math->Substract) does not work for me, since
the pixel intensities in ImageA are only reduced by values of ImageB
pixels. Can I use imageJ standard operations for this purpose or do I
need a plugin? Any help is greatly appreciated. Thanky you in advance.

Cheers
Oliver