Posted by
Sasmita Rath on
Jun 02, 2008; 2:08pm
URL: http://imagej.273.s1.nabble.com/FRET-calculation-tp3696076p3696077.html
Thank you very much for your reply.I do stimulated acceptor emission(Tag protein with GFP-YFP and excite using laser to find the FRET ) .Yes you are right .Its not possible to use FRET plugins's methods in macros .I tried and failed.
Could you please let me know the name of the plugins and the (working version to process stacks) you mentioned.
Sasmita
----- Original Message -----
From: "George Sharonov" <
[hidden email]>
To:
[hidden email]
Sent: Monday, June 2, 2008 2:21:14 AM GMT -06:00 US/Canada Central
Subject: Re: FRET calculation .
I'm working with FRET too. I'm measuring stimulated acceptor emission within
time stacks. There are two plugins for measurement of stimulated emission,
but they are not intended to process stacks. Therefore I'm writing my own
plugin that allows to process stacks (working version is available if you
need).
I'm not familiar with macro language but I believe that it is almost
impossible to use FRET plugins's methods in macros. Thus it is better to use
plugin as it is. If you need to modify plugin you need to modify its source
code.
What kind of FRET experiments do you have, photobleaching or stimulated
emission?
On Mon, May 26, 2008 at 2:32 AM, Sasmita Rath <
[hidden email]> wrote:
> Hello All,
> I'm a new user of imageJ. I'm writting some macros in order to accomplish
> my
> calculations.I do pixel by pixel FRET calculation.I found the plugin
> named"FRET
> calc v3.0 plugin for analysis of FRET by acceptor photobleaching" written
> by
> David Stepensky.
>
> Could I use that program to do my analysis ?I'm asking because I tried to
> use
> that and I found some error message.I know little about java programs.Is it
> a
> good way to write my own macros in java or is it ok to use the programs
> already
> available.
>
> I'm a bit confused.I appreciate any suggestion.
>
George Sharonov,
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Moscow, Russia