Image processing

Posted by degussa on
URL: http://imagej.273.s1.nabble.com/Image-processing-tp3696122.html

. I am really struggling  with  image addition and subtraction given me noisy / irreasonable results. I have three images (a) the reference image( dry  voids) (b) the time series image( partially filed void) (c) The saturated image( completely filled void). I perform the following  arithmetic

1. subtract image A from B to quantify the amount of  fluid in void after  a certain time
2. subtract image A from C to qualify the  voids within the images
3. I ratio the result from 1 to 2 to quantify the realative concetration of fluid in voids at time of acquiring image 2.

Problem
. When i do my subtraction, i  end up having negative pixels, whioch should not occur, how do i eliminate these pixels without compromising my  data

When i ratio  as described in step 3 above, i get values   above 1 which is not logical,  how do i remove values greater than 1 without compromising my data.  I attach 3 images  for anyone to please help me out. Thanks href="Reference-+dry+void.tif">Reference-+dry+void.tif8+hours+partially+filled+voids.tifSaturated-filled+voids.tif