Posted by
Han Gang Yu on
URL: http://imagej.273.s1.nabble.com/Image-processing-tp3696122p3696123.html
Almost done:
1) ST 5uM? turn off your Chinese software.
2) why a box on the black line for control?
3) replace 1000pA with 1 nA
HG
>>> On 5/28/2008 at 12:26 AM, in message <
[hidden email]>, degus <
[hidden email]> wrote:
. I am really struggling with image addition and subtraction given me noisy
/ irreasonable results. I have three images (a) the reference image( dry
voids) (b) the time series image( partially filed void) (c) The saturated
image( completely filled void). I perform the following arithmetic
1. subtract image A from B to quantify the amount of fluid in void after a
certain time
2. subtract image A from C to qualify the voids within the images
3. I ratio the result from 1 to 2 to quantify the realative concetration of
fluid in voids at time of acquiring image 2.
Problem
. When i do my subtraction, i end up having negative pixels, whioch should
not occur, how do i eliminate these pixels without compromising my data
When i ratio as described in step 3 above, i get values above 1 which is
not logical, how do i remove values greater than 1 without compromising my
data. I attach 3 images for anyone to please help me out. Thanks
href="Reference-+dry+void.tif">Reference-+dry+void.tif
http://www.nabble.com/file/p17505032/8%2Bhours%2Bpartially%2Bfilled%2Bvoids.tif
8+hours+partially+filled+voids.tif
http://www.nabble.com/file/p17505032/Saturated-filled%2Bvoids.tif
Saturated-filled+voids.tif
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