Image analysis of aggregates in a liquid medium
Posted by Kashif Zeeshan on May 06, 2008; 2:06pm
URL: http://imagej.273.s1.nabble.com/Image-analysis-of-aggregates-in-a-liquid-medium-tp3696326.html
HI,
I am a new user of ImageJ (I am using MacBiophotonic ImageJ upgraded to
version 1.41a) and I am trying to practice the image analysis to quantify
the aggregates present in liquid medium. But I am facing a lot of problems.
Sometimes, the threshold it works and sometimes it doesnt. I try to explain
a little bit the procedure. I pour the medium with aggregates into a large
Petri dish and then I take photo with a dark blue background by an ordinary
digital camera (Panasonic, DMC-LS2, 5 Mega Pixels) in default mode without
flash. I have a problem of reflection of light. After taking photos, I
transfer them to the computer and launch ImageJ. In ImageJ, I open the
selected image and then I use the toolbar button RGB-Merge/Split to have
the 3 different images (transferred into red, blue and green. I keep the red
one for further processing. On that red image, I apply the Segmentation
plugin Otsu Thresholding 8bit. After that I use the command
Analyze>Analyze Particles
. It worked for one assay but not working now. I
am blocked at this stage. Can you kindly give me some suggestion to improve
the methodology or how I can progress with this?
Cordially.
Kashif ZEESHAN
(00 33 6 74 90 18 10)
Doctorant, Biopesticide Group,
Laboratoire Universitaire de
Biodiversité et d'Ecologie
Microbienne (LUBEM),
6 Rue de l'Université,
29334, Quimper Cedex,
France