Particle Tracker

Posted by jason li-4 on
URL: http://imagej.273.s1.nabble.com/Particle-Tracker-tp3696333.html

Hi,

   I was wondering if anyone has run into any trouble using the Particle
Tracker. The program is not detecting any of my cells; specifically, it
produces random dots rather than circling the cells. I thought it was a
threshold problem because my cells are black on a white background, but
even after tweaking the threshold and subtracting the background it did not
work. Does anyone have any advice? Thanks for your time and help.

                                    Sincerely,
                                       Jason Li