counting of colocation area

Posted by agus wijoyo on Apr 28, 2008; 9:05am
URL: http://imagej.273.s1.nabble.com/counting-of-colocation-area-tp3696372.html

Dear Forum

I would like to find out the location of my protein in the cells, the protein was stained with Red Fluorescent Protein, and located in both the cytoplasm and nucleus. The nucleus of the cells was stained with DAPI (blue colour). After merge the 2 image, there were part of protein which was located in the nucleus, and make colocation with the blue colour of the nucleus. I want to count the percentage of the protein in the nucleus area and in the cytoplasm. Please give me an advise

 

Thank you

Gus
 




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