Re: MEB width length diameter measurement

Posted by Unruh, Jay on Apr 25, 2008; 1:30pm
URL: http://imagej.273.s1.nabble.com/MEB-width-length-diameter-measurement-tp3696446p3696447.html

Olivia,

Please see Raub et. al., Biophys. J., (2008), Vol. 94, p. 2361.  Two dimensional image autocorrelation will give you a good estimate of the fiber diameters.  The length is a much more difficult proposition.  In order to  autocorrelate in ImageJ, use Process-FFT-FDmath and choose correlate from the middle menu.

Jay

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Bouvard, Olivia
Sent: Friday, April 25, 2008 1:29 AM
To: [hidden email]
Subject: MEB width length diameter measurement

Hi all,



I am very new to imageJ for the moment I'm doing a counting of fiber diameter manually and I would like to automate it.

The image represents fibers on a filter, I need to measure their diameter which is actually the apparent width. Also if possible I would like to know their length but the fiber is not straight, and many fibers can be crossing.

Do you think this is possible using imageJ ? and if yes, how ?



Regards,



Olivia