Colour extraction using Image J
Posted by amhaughey on Apr 18, 2008; 3:37pm
URL: http://imagej.273.s1.nabble.com/Colour-extraction-using-Image-J-tp3696534.html
Hi,
I have been using Image J to analyse images of a fluorescent molecule taken in
ambient light (it is not possible to capture the images in complete darkness).
In the images that are obtained it is impossible to see the fluorescent light.
So far I have been taking an image of the source when there is fluorescence
present and then taking a second image after the fluorescence has decayed. I
then use Image J to subtract the two images. This has been working well, I can
see the fluorescent light. However, things like reflections interfere with the
results as these can show up as bright patches in the subtracted image masking
the true results.
Ideally I would like to be able to extract the light (wavelength, or waveband)
of interest from the image. Is there a way of doing this with Image J?
I would greatly appreciate any help that anyone can offer or suggestions for
another type of software that could do this if Image J does not have these
capibilities.
Thanks,
Anne-Marie