Posted by
Joachim Wesner on
Apr 19, 2008; 7:58am
URL: http://imagej.273.s1.nabble.com/Colour-extraction-using-Image-J-tp3696534p3696536.html
Hi there,
I think the original poster was more likely interested in a
software-only/mostly solution!
However, some color-support in hardware needs to be there. If you have a
color image/camera,
there was an announcement of a "color deconvolution plugin" sometime ago in
this
forum, that can extract certain "color patterns" from images IIRC.
You should searchh the forum or look in the plugins dirctory.
https://list.nih.gov/cgi-bin/wa?S1=imagejhttp://rsb.info.nih.gov/ij/plugins/In case of a monochrom camera, no luck!
.
Hope this helps!
Cheers, Joachim
ImageJ Interest Group <
[hidden email]> schrieb am 18.04.2008 19:02:49:
> Is it possible to attach a band pass filter to the camera lens that
> blocks all frequencies other than those associated with the fluorescence?
> Can higher intensity images be obtained using extended integration time
> for the camera? A polarizing filter may reduce reflections.
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf
> Of amhaughey
> Sent: Friday, April 18, 2008 11:37 AM
> To:
[hidden email]
> Subject: Colour extraction using Image J
>
> Hi,
>
> I have been using Image J to analyse images of a fluorescent molecule
taken
> in
> ambient light (it is not possible to capture the images in complete
> darkness).
> In the images that are obtained it is impossible to see the fluorescent
> light.
> So far I have been taking an image of the source when there is
fluorescence
> present and then taking a second image after the fluorescence has
decayed.
> I
> then use Image J to subtract the two images. This has been working well,
I
> can
> see the fluorescent light. However, things like reflections interfere
with
> the
> results as these can show up as bright patches in the subtracted image
> masking
> the true results.
>
> Ideally I would like to be able to extract the light (wavelength, or
> waveband)
> of interest from the image. Is there a way of doing this with Image J?
>
> I would greatly appreciate any help that anyone can offer or suggestions
for
> another type of software that could do this if Image J does not have
these
> capibilities.
>
> Thanks,
>
> Anne-Marie
> --
> View this message in context:
http://www.nabble.com/Colour-> extraction-using-Image-J-tp16763514p16763514.html
> Sent from the ImageJ mailing list archive at Nabble.com.
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