Problem with olympus file format

Posted by Mickael Lefebvre on Apr 16, 2008; 8:18am
URL: http://imagej.273.s1.nabble.com/Problem-with-olympus-file-format-tp3696550.html

Hi !

I use and recommend ImageJ to my customers for open and read olympus
microscope's image format (OIB and OIF). Nevertheless I encounter a
problem : my image has 3 channels, corresponding to DAPI, Alexa 488 and
mito tracker. When I visualise it with Olympus fluoview viewer, the colors
are correctly attributed to each channel, but when I use ImageJ to open
it, some colors are inverted, like DAPI appear red and mito tracker blue.
I have tried different plugins to solve this problem, but I haven't find
solution.

Could somebody help me ?

Thanks.

Mickaël Lefebvre
Olympus France