Re: Problem with olympus file format

Posted by Michael Weber-4 on Apr 16, 2008; 11:24am
URL: http://imagej.273.s1.nabble.com/Problem-with-olympus-file-format-tp3696550p3696551.html

Hi Mickaël,

same happens to other file formats such as Leica lif while using the
LOCI importer, the LUT cannot be assigned correctly. However, if you
want to analyze something, color doesn't matter, if you need the
overlay, RGB merge will assign the color you want. In any other case,
you can manually assign a LUT for a single channel.

Michael


Mickael Lefebvre wrote:

> Hi !
>
> I use and recommend ImageJ to my customers for open and read olympus
> microscope's image format (OIB and OIF). Nevertheless I encounter a
> problem : my image has 3 channels, corresponding to DAPI, Alexa 488 and
> mito tracker. When I visualise it with Olympus fluoview viewer, the colors
> are correctly attributed to each channel, but when I use ImageJ to open
> it, some colors are inverted, like DAPI appear red and mito tracker blue.
> I have tried different plugins to solve this problem, but I haven't find
> solution.
>
> Could somebody help me ?
>
> Thanks.
>
> Mickaël Lefebvre
> Olympus France