?? On quantification of Western blot bands using ImageJ

Posted by Zhigang on Apr 07, 2008; 3:52pm
URL: http://imagej.273.s1.nabble.com/On-quantification-of-Western-blot-bands-using-ImageJ-tp3696585.html

Dear ImageJ Expert colleagues,

I would like to quantify the protein bands of western blot/SuperSignal
ChemiLuminescent (Pierce) using ImageJ.

It will be very appreciated, if you could send me the standard procedure to
get this done.

Detailed information on how to calibrate the bands in order to keep the data
in the linear range will be very helpful.

Any suggestions on reference papers using ImageJ as the method for
quantification of Western-blot data is very welcomed.

Thanks for your help

Zhigang
---------------------------
Zhigang Kang  Research Fellow
Section on Neuroendocrine Immunology and Behavior
Intergrative Neural Immune Program, NIH
5625 Fishers Lane, Room 4N15, MSC-9401,
Rockville MD 20852
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