Re: ?? On quantification of Western blot bands using ImageJ

Posted by Eddie Iannuccelli on Apr 07, 2008; 4:32pm
URL: http://imagej.273.s1.nabble.com/On-quantification-of-Western-blot-bands-using-ImageJ-tp3696585p3696588.html

Try the Analyse->Gel menu

Here is a small tutorial forone-dimensional electrophoretic gels :
http://rsb.info.nih.gov/nih-image/manual/tech.html#analyze
We used a linear model (in excel) with dna control bands to quantify our
dna samples from agarose gels, don't know if you can use such linear
model for your protein gel . Another tutorial here for dot blots :
http://rsbweb.nih.gov/ij/docs/examples/dot-blot/

Regards

Zhigang a écrit :

> Dear ImageJ Expert colleagues,
>
> I would like to quantify the protein bands of western blot/SuperSignal
> ChemiLuminescent (Pierce) using ImageJ.
>
> It will be very appreciated, if you could send me the standard procedure to
> get this done.
>
> Detailed information on how to calibrate the bands in order to keep the data
> in the linear range will be very helpful.
>
> Any suggestions on reference papers using ImageJ as the method for
> quantification of Western-blot data is very welcomed.
>
> Thanks for your help
>
> Zhigang
> ---------------------------
> Zhigang Kang  Research Fellow
> Section on Neuroendocrine Immunology and Behavior
> Intergrative Neural Immune Program, NIH
> 5625 Fishers Lane, Room 4N15, MSC-9401,
> Rockville MD 20852
> Telephone:[phone]
> Fax:[phone]
> Email:[hidden email]
>    
>  

--
******************************************
Eddie Iannuccelli
Laboratoire de génétique cellulaire
INRA - Castanet Tolosan
Tel: [phone] / Fax: [phone]