Posted by
Rigo Pantoja on
Apr 10, 2008; 8:14pm
URL: http://imagej.273.s1.nabble.com/plot-profile-problems-with-pixel-value-tp3696589p3696591.html
Hi Harry,
Wayne recommened your suggestion. It did not work.
Basically, when you zoom in and draw a line across a set of linear pixels,
you get a variable reading of the pixel value if the line does not cross the
pixel in the exact center. If your pixels values are high you might not
notice. However, if you are analyzing single-molecues - where every count
matters - this becomes obvious.
If you follow the thread you can see this effect with a sample image
(fluorescentcells), which after you convert it to a 16bit tiff file.
Thanks,
Rigo
On Thu, Apr 10, 2008 at 10:34 AM, Harry Parker <
[hidden email]>
wrote:
> Have you tried deselecting the menu option, Edit->Options->Profile Plot
> Options...->Interpolate Line Profiles?
>
> --
> Harry Parker
> Senior Systems Engineer
> Digital Imaging Systems, Inc.
>
> ----- Original Message ----
> From: Rigo Pantoja <
[hidden email]>
> To:
[hidden email]
> Sent: Wednesday, April 9, 2008 2:35:21 PM
> Subject: plot profile problems with pixel value
>
> All,
>
> The plot profile (Ctrl+K) is yielding different values for the same
> pixel.
> I Noticed this when I generated plot profiles for an individual spot along
> the x and y axis. The peak pixel value was different for the comon
> interesection. Also, if you generate a various plot profiles for with
> increasing lengths, the peak pixel value changes. Can this bug be fixed?
>
> Thanks,
>
> Rigo
>
>
>
>
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