http://imagej.273.s1.nabble.com/plot-profile-problems-with-pixel-value-tp3696589p3696593.html
The problem is fixed in version 1.40e. Thanks,
> I think the problem you're seeing arises from the spatial definitions
> and representations of each pixel. Rather than go through a lengthy
> explanation, try using the point selection tool and Measurements
> (which will give the same results as Plot Profile and is faster to
> check) when zoomed in. You'll see the effect causing your results,
> which actually arises from a very methodical way of processing the
> pixelated data.
>
> The slight difference I see between the point selection tool and line
> selections is that the line selections seems to go to the nearest
> pixel "corner" for a measurement while the point selection is always
> to the upper-left.
>
> Jonathan
>
>
> On Thu, Apr 10, 2008 at 2:14 PM, Rigo Pantoja <
[hidden email]>
> wrote:
> > Hi Harry,
> >
> > Wayne recommened your suggestion. It did not work.
> >
> > Basically, when you zoom in and draw a line across a set of linear
> pixels,
> > you get a variable reading of the pixel value if the line does not
> cross the
> > pixel in the exact center. If your pixels values are high you might
> not
> > notice. However, if you are analyzing single-molecues - where every
> count
> > matters - this becomes obvious.
> >
> > If you follow the thread you can see this effect with a sample image
> > (fluorescentcells), which after you convert it to a 16bit tiff file.
> >
> > Thanks,
> >
> > Rigo
> >
> > On Thu, Apr 10, 2008 at 10:34 AM, Harry Parker <
[hidden email]>
> > wrote:
> >
> >
> >
> > > Have you tried deselecting the menu option, Edit->Options->Profile
> Plot
> > > Options...->Interpolate Line Profiles?
> > >
> > > --
> > > Harry Parker
> > > Senior Systems Engineer
> > > Digital Imaging Systems, Inc.
> > >
> > > ----- Original Message ----
> > > From: Rigo Pantoja <
[hidden email]>
> > > To:
[hidden email]
> > > Sent: Wednesday, April 9, 2008 2:35:21 PM
> > > Subject: plot profile problems with pixel value
> > >
> > > All,
> > >
> > > The plot profile (Ctrl+K) is yielding different values for the same
> > > pixel.
> > > I Noticed this when I generated plot profiles for an individual spot
> along
> > > the x and y axis. The peak pixel value was different for the
> comon
> > > interesection. Also, if you generate a various plot profiles for
> with
> > > increasing lengths, the peak pixel value changes. Can this bug be
> fixed?
> > >
> > > Thanks,
> > >
> > > Rigo
> > >
> > >
> > >
> > >
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> >
>