Color profile for calcium waves
Posted by
Syla on
Apr 09, 2008; 3:10am
URL: http://imagej.273.s1.nabble.com/Color-profile-for-calcium-waves-tp3696594.html
Hello all !
I am beginning to use ImageJ, and I would like to use it to analyse calcium waves in neurons and astrocytes. I acquire stack of images along time, and I can use a ROI to measure fluorescence intensity and make a graph of this intensity over time. But I would like to represent this profile by a color graph, a bit like the 'Line scanning' of Olympus Fluoview software. Shortly speaking, is there a plugin or macro which permit to draw a ROI (line would be better), to get the pixels intensities under this line, and to plot it over time in a 2D graph ?
It should give somethink like this: X-axis is the line profile, Y-Axis is the time.
http://uppix.net/f/b/7/5414812d435051dd24240b31f8dad.jpgWith the 'Dynamic Profiler' I have the first part of this but I couldn't find how to plot it 'graphically'. 'MultiMeasure' gives only graphs and works with areas (and then gives an average, which is less useful for me).
Thank you !