Sperm Analysis

Posted by Taufiq Purna Nugraha on Apr 07, 2008; 3:08pm
URL: http://imagej.273.s1.nabble.com/Sperm-Analysis-tp3696629.html

Dear All,

I'm learning to use image to analyze sperm sample, mainly to measure
head area, head width & length and also tail length.
I know this subject has been discussed quite for some time but I still
have some difficulties.  For the head area I'm using
particle analysis (PA) to measure the area, but the main problem is
how to accurately separate between head and tail (to
measure head area). Because in the PA I define the sperm head as a
circular object with circularity between 0.75-0.9,
I have try several methods to separate the tail from the head using
such as erode and dilate or minimum and maximum filter
and the main problem of this method is the sperm sometimes became to
round since the dilate or maximum filter only
interpolate from the eroded condition.  The best method so far to cut
the tail from the head manually using the pen tools.

Is there anyway to calculate head area without the necessity to
separate head from tail ?  For the head length I'm using ferrets
from ImageJ PA, but for the sperm width (breadth) I still having the
difficulties to count it automatically.  And is there any way to
measure
tail length automatically ? So far I'm using manual methods using line
tools but sometimes became very  frustrating when I have to measure
3000 cells.

For the sample image of the sperm I upload it at
http://picasaweb.google.com/RTP.Nugraha/Sperm/photo#5186514543892861554

Thanks in advance and looking forward to hear any suggestion

--
R. Taufiq Purna Nugraha, DVM.

Laboratory of Reproduction
Div. of Zoology [Museum Zoologicum Bogoriense]
R.C. for Biology
Indonesian Institute of Sciences [LIPI]
Jl. Raya Jakarta – Bogor Km. 46, Cibinong 16911
INDONESIA
Telp : [phone]/64 Ext. 151
Fax : [phone]
e-mail : [hidden email], [hidden email]