Re: Sperm Analysis

Posted by Taufiq Purna Nugraha on Apr 08, 2008; 1:43pm
URL: http://imagej.273.s1.nabble.com/Sperm-Analysis-tp3696629p3696631.html

Dear Karsten and Chris

Thanks for the advice, I try the gray morphology plugins last night, but
this method still have a bias just like the minimum and maximum filter,
where the sperm became to round and over estimate on the head and tail
junction, I will try again on different samples.  And for Chris  I will try
your advice tonight.

btw, is the gray morphology that slow ?, I use it on an image with 2816 x
2112 pixel resolution and it takes more than one minutes to produce the
result.  And do you have any advice how to count the sperm tail length
automatically ?

Cheers,



On Mon, Apr 7, 2008 at 10:44 PM, Karsten Rodenacker <[hidden email]>
wrote:

> Try Morphology plugin "graymorphology, radius 10., circle, close" on the
> gray image. You have than the sperm heads without tail for particle
> analysis.
>
> Regards
> Karsten
>
> Am 07.04.2008 um 17:08 schrieb Taufiq Purna Nugraha:
>
>  For the sample image of the sperm I upload it at
> > http://picasaweb.google.com/RTP.Nugraha/Sperm/photo#5186514543892861554
> >
>

--
R. Taufiq Purna Nugraha, DVM.

Laboratory of Reproduction
Div. of Zoology [Museum Zoologicum Bogoriense]
R.C. for Biology
Indonesian Institute of Sciences [LIPI]
Jl. Raya Jakarta – Bogor Km. 46, Cibinong 16911
INDONESIA
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