Re: Sperm Analysis

Posted by karo03 on Apr 08, 2008; 2:51pm
URL: http://imagej.273.s1.nabble.com/Sperm-Analysis-tp3696629p3696632.html

Am 08.04.2008 um 15:43 schrieb Taufiq Purna Nugraha:

> Dear Karsten and Chris
>
> Thanks for the advice, I try the gray morphology plugins last night,  
> but
> this method still have a bias just like the minimum and maximum  
> filter,
> where the sperm became to round and over estimate on the head and tail
> junction, I will try again on different samples.  And for Chris  I  
> will try
> your advice tonight.
>
> btw, is the gray morphology that slow ?, I use it on an image with  
> 2816 x
> 2112 pixel resolution and it takes more than one minutes to produce  
> the
> result.  And do you have any advice how to count the sperm tail length
> automatically ?

Computing time is dependent on the machine and the radius chosen. In  
fact it is slow.
The difference between the original and the closed image allows to  
detect the sperm tails quite well. Possibly with a Threshold, another  
(binary) opening (small) and a skeleton. For each connected object  
(isolated tail) the number of pixels is an estimate, not good but  
possibly sufficient. Another one possibly better is the perimeter/2 of  
each connected skeletonized object.
Karsten

>
> Cheers,
>
>
>
> On Mon, Apr 7, 2008 at 10:44 PM, Karsten Rodenacker <[hidden email]
> >
> wrote:
>
>> Try Morphology plugin "graymorphology, radius 10., circle, close"  
>> on the
>> gray image. You have than the sperm heads without tail for particle
>> analysis.
>>
>> Regards
>> Karsten
>>
>> Am 07.04.2008 um 17:08 schrieb Taufiq Purna Nugraha:
>>
>> For the sample image of the sperm I upload it at
>>> http://picasaweb.google.com/RTP.Nugraha/Sperm/photo#5186514543892861554
>>>
>>
>
>
> --
> R. Taufiq Purna Nugraha, DVM.
>
> Laboratory of Reproduction
> Div. of Zoology [Museum Zoologicum Bogoriense]
> R.C. for Biology
> Indonesian Institute of Sciences [LIPI]
> Jl. Raya Jakarta – Bogor Km. 46, Cibinong 16911
> INDONESIA
> Telp : [phone]/64 Ext. 151
> Fax : [phone]
> e-mail : [hidden email], [hidden email]