RV: Question for 8-bit grayscale values

Posted by pvizan on Mar 26, 2008; 5:11pm
URL: http://imagej.273.s1.nabble.com/RV-Question-for-8-bit-grayscale-values-tp3696737.html

Dear all, we have a question for quantification methods of ImageJ, could
anybody help us?

 

For an optical density calibration, when you are capturing 8-bit grayscale
images (values from 0-256), it is supposed that the darkest standard picture
corresponds to the lowest value. In our case that is not true, the darkest
standard picture is shown as the clearest picture and the mean value is the
highest. It seems that the camera is acquiring the picture in “negative
mode”. Does it make any sense? Does anybody know how to change/solve it?

 

Thank you very much in advance

 

Pedro

 

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Pedro Vizán, PhD.

Departament of Biochemistry and Molecular Biology

Institute of Biomedicine of University of Barcelona

phone number: 0034 934021217

e-mail: [hidden email]