Second question for 8-bit grayscale values

Posted by pvizan on Mar 27, 2008; 4:03pm
URL: http://imagej.273.s1.nabble.com/RV-Question-for-8-bit-grayscale-values-tp3696737p3696739.html

Dear all,


For an optical density calibration, when you are capturing 8-bit grayscale
images (values from 0-256), it is supposed that the darkest standard picture
corresponds to the lowest value (black=0/white=255 convention), but in our
case we have a black=255/white=0 convention. Does anybody know how to modify
the acquisition process to invert the O.D. values? Using "Edit -> Invert"
does not change O.D. values.

Pedro

PS: We are using a Leica DM4000B digital microscopy. For image capturing a
monochrome digital camera Scion CFW-1312M is being used.

--

Pedro Vizán, PhD.
Departament of Biochemistry and Molecular Biology
Institute of Biomedicine of University of Barcelona
phone number: 0034 934021217
e-mail: [hidden email]