Antwort: Second question for 8-bit grayscale values

Posted by Joachim Wesner on Mar 31, 2008; 11:17am
URL: http://imagej.273.s1.nabble.com/RV-Question-for-8-bit-grayscale-values-tp3696737p3696740.html

ImageJ Interest Group <[hidden email]> schrieb am 27.03.2008 17:03:11:

> Dear all,
>
>
> For an optical density calibration, when you are capturing 8-bit
grayscale
> images (values from 0-256), it is supposed that the darkest standard
picture
> corresponds to the lowest value (black=0/white=255 convention), but in
our
> case we have a black=255/white=0 convention. Does anybody know how to
modify
> the acquisition process to invert the O.D. values? Using "Edit -> Invert"
> does not change O.D. values.
>
> Pedro
>

Pedro,

easy, you should be able to use "Process/Math/XOR" with 255

Joachim


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