Posted by
Kenneth Sloan-2 on
Nov 07, 2007; 8:54pm
URL: http://imagej.273.s1.nabble.com/Loosing-Particles-when-Rotating-Image-tp3696797p3696802.html
Excuse my ignorance of IJ internals (I'm just starting to use it) -
but is it not possible to find ALL particles and then catagorize each
one according to which ROI it lies in? What I would look for is a
routine that produced an array (or list) of particles annotated with
x,y coordinates. It would then be simple to step through that list
and produced a histogram of #particles per angular bin.
Of course, rotating the image is blurring the particles and merging
them. You could minimize the problems by always rotating from the
same starting image. Even then you would get some loss, but the loss
would not depend on how far you rotated. Rotating incrementally is a
classic error.
On Nov 7, 2007, at 12:38 PM, Gabriel Landini wrote:
> On Wednesday 07 November 2007, Brendan H. wrote:
>> 1) When I rotate the image arbitrarily without iterating, I loose
>> a bunch
>> of particles each time.
>
> Of course, you are rotating a square pixel array by a 5 degree angle!
>
> Your only option is to rotate the ROI.
>
> G.
--
Kenneth Sloan
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Computer and Information Sciences +1-205-934-2213
University of Alabama at Birmingham FAX +1-205-934-5473
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http://KennethRSloan.com/