Re: Contrast applies to the entire stack?

Posted by Wayne Rasband on Mar 19, 2008; 2:00pm
URL: http://imagej.273.s1.nabble.com/Contrast-applies-to-the-entire-stack-tp3696822p3696823.html

> Why must the settings of the Adjust>Brightness/Contrast dialog affect
> an entire
> image stack? Is there any way around this?  I have a set of 3 images
> which have
> different min/max pixel values, and I would like to animate and
> analyze them as
> a stack, but there is no single contrast setting that works for all
> three.  I would
> rather not fiddle with the values of the pixels themselves, I consider
> this a
> display issue..

Use the Image>HyperStacks>Stack to HyperStack command to convert the
stack to a hyperstck. In the dialog box, set "Channels" to the number
of images in the stack and "Display Mode" to "Grayscale". In a
hyperstack, each channel has its own brightness and contrast settings.

-wayne