nuclei intensity in a timelapse

Posted by F Javier Díez Guerra on Mar 14, 2008; 2:49pm
URL: http://imagej.273.s1.nabble.com/nuclei-intensity-in-a-timelapse-tp3696875.html

Hello,

I want to measure the average intensity of cell nuclei from a
timelapse (aprox 400 frames) with 2-3 cells expressing a GFP fusion
that enters and leaves the nucleus. The cells are moving and the
nuclei also move and change shape.

In the end, each frame should be thresholded to obtain 2-3 ROIs
outlining cell nuclei where average intensity could be measured. Any
idea on how to automate this task?

Thanks,