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Re: Subtract Background Help

Posted by NeLaS on Feb 27, 2008; 12:33pm
URL: http://imagej.273.s1.nabble.com/Subtract-Background-Help-tp3697080p3697082.html

>
> I was wondering if there is a way of changing the default colour once

the subtract background function has been used.
>
> At the moment it is set to black, and as I am looking at some dark
> material it would be useful for me if the background was white not
> black.
>


Hey Rhodri,

Maybe I did not understand what you mean, but isn't a matter of ticking the
"light background" box in the  subtract background dialog? Then you will get
a white background.

What version of ImageJ are you using?

cheers,

bruno


On Wed, Feb 27, 2008 at 7:59 AM, Joachim Wesner <
[hidden email]> wrote:

> Hi Rhodri,
>
> you can invert the whole image ("Invert LUT" or use other image
> processing)
>
> IMHO only replacing one color out of 256 (or even up to 65536) in the LUT
> is probably not what you want, if your background is not 100% flatr and
> noisy, it will not work as you expect.
>
> Maybe I do not understand what exactly "default color" means in your
> case.....
>
> Mit freundlichen Grüßen / Best regards
>
> Joachim Wesner
>
>
>
>
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>             27.02.2008 11:41
>
>
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>
>
> Hi there,
>
>
>
> I was wondering if there is a way of changing the default colour once
> the subtract background function has been used.
>
> At the moment it is set to black, and as I am looking at some dark
> material it would be useful for me if the background was white not
> black.
>
>
>
> Thanking you for your help in advance.
>
>
>
> Rhodri Wilson
>
>
>
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