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Subtracting Background Problem

Posted by Naveen Krishnan on Feb 19, 2008; 5:16pm
URL: http://imagej.273.s1.nabble.com/Subtracting-Background-Problem-tp3697129.html

I have sets of images that vary in overall background intensity and I was
wondering if there was a quick fix that could normalize the backgrounds of
all the images to zero (or close to zero), while removing the corresponding
artificial intensity from the cells in the images. I have used the subtract
background function of imageJ with various parameters for the rolling ball
radius but I am wondering if there is a more accurate way. I have also tried
some of the plugins on the internet for background correction but so far
with the parameters I have used I haven't seen any good results. Any help
here would be greatly appreciated.

Thanks,

Naveen