Re: Subtracting Background Problem
Posted by
Ralph Common on
Feb 20, 2008; 3:10am
URL: http://imagej.273.s1.nabble.com/Subtracting-Background-Problem-tp3697129p3697130.html
There is a free, easy to use program called Image Arithmetic that will do
the job. It can be downloaded from
http://www.t3i.nl/myblog/?page_id=7Ralph Common
----- Original Message -----
From: "Naveen Krishnan" <
[hidden email]>
To: <
[hidden email]>
Sent: Tuesday, February 19, 2008 12:16 PM
Subject: Subtracting Background Problem
> I have sets of images that vary in overall background intensity and I was
> wondering if there was a quick fix that could normalize the backgrounds of
> all the images to zero (or close to zero), while removing the
corresponding
> artificial intensity from the cells in the images. I have used the
subtract
> background function of imageJ with various parameters for the rolling ball
> radius but I am wondering if there is a more accurate way. I have also
tried
> some of the plugins on the internet for background correction but so far
> with the parameters I have used I haven't seen any good results. Any help
> here would be greatly appreciated.
>
> Thanks,
>
> Naveen
>