Re: Subtracting Background Problem

Posted by Naveen Krishnan on Feb 20, 2008; 4:05pm
URL: http://imagej.273.s1.nabble.com/Subtracting-Background-Problem-tp3697129p3697135.html

Were you referring to performing subtractions using the mask of a background
as image2 and the original image as image1 or do you know a better way to
subtract out the background for a large number of images using this program?

Thanks,

Naveen

On Tue, Feb 19, 2008 at 10:10 PM, Ralph Common <[hidden email]> wrote:

> There is a free, easy to use program called Image Arithmetic that will do
> the job.  It can be downloaded from
>
> http://www.t3i.nl/myblog/?page_id=7
>
> Ralph Common
>
> ----- Original Message -----
> From: "Naveen Krishnan" <[hidden email]>
> To: <[hidden email]>
> Sent: Tuesday, February 19, 2008 12:16 PM
> Subject: Subtracting Background Problem
>
>
> > I have sets of images that vary in overall background intensity and I
> was
> > wondering if there was a quick fix that could normalize the backgrounds
> of
> > all the images to zero (or close to zero), while removing the
> corresponding
> > artificial intensity from the cells in the images. I have used the
> subtract
> > background function of imageJ with various parameters for the rolling
> ball
> > radius but I am wondering if there is a more accurate way. I have also
> tried
> > some of the plugins on the internet for background correction but so far
> > with the parameters I have used I haven't seen any good results. Any
> help
> > here would be greatly appreciated.
> >
> > Thanks,
> >
> > Naveen
> >
>