Re: ImageJ - Vesicle movement analysis

Posted by Martin Jaekel on Feb 20, 2008; 8:24am
URL: http://imagej.273.s1.nabble.com/ImageJ-Vesicle-movement-analysis-tp3697136p3697137.html

Hi Tal,

you could maybe use the watershed algorithm to separate those close
objects. you can find the watershed function in one of the process menu
options i think.

best,
martin

On Feb 20 2008, Tal Shprung wrote:

>Dear all,
>
>I apologize if i repeat a previously asked question, but i was not sure how
>to search for the solution.
>
>I load to the program two consecutive images (10sec apart) of a live cell
>with its vesicle fluorescently painted.
>
>what i get is two images with many vesicles (round shaped objects).
>
>what i need is to end up with some kind of number to describe the movement
>of the vesicle (small movement/ short or long distance/
>
>a fraction of the vesicles moving)
>
>What i currently do is turn the pictures to Binary and adjust threshold to
>auto and then subtract the second picture from the first.
>
>I measure the area resulted and divide that from the first picture (time
>0sec).
>
>My problem is that by turning the picture to Binary, some close vesicles
>appear as one big object.
>
>How can i separate these vesicles? does anyone has a suggestion on a
>different way of analysis?
>
>Thank you very much.
>
>Tal Shprung
>
>P.S.
>
>I want to thank the people behind this wonderful (and free) program. You
>have made my research possible.
>