Re: ImageJ - Vesicle movement analysis

Posted by Tal Shprung on Feb 20, 2008; 12:08pm
URL: http://imagej.273.s1.nabble.com/ImageJ-Vesicle-movement-analysis-tp3697136p3697138.html

Hi Martin,

The watershed is not enough, it does seperate the objects but very slightly.

I am looking for something that, still in the colored picture, will find the
strongest points within each vesicle's center of mass and decress the
vesicle's size a bit, to seperate the vesicles.

On Feb 20, 2008 10:24 AM, M. Jaekel <[hidden email]> wrote:

> Hi Tal,
>
> you could maybe use the watershed algorithm to separate those close
> objects. you can find the watershed function in one of the process menu
> options i think.
>
> best,
> martin
>
> On Feb 20 2008, Tal Shprung wrote:
>
> >Dear all,
> >
> >I apologize if i repeat a previously asked question, but i was not sure
> how
> >to search for the solution.
> >
> >I load to the program two consecutive images (10sec apart) of a live cell
> >with its vesicle fluorescently painted.
> >
> >what i get is two images with many vesicles (round shaped objects).
> >
> >what i need is to end up with some kind of number to describe the
> movement
> >of the vesicle (small movement/ short or long distance/
> >
> >a fraction of the vesicles moving)
> >
> >What i currently do is turn the pictures to Binary and adjust threshold
> to
> >auto and then subtract the second picture from the first.
> >
> >I measure the area resulted and divide that from the first picture (time
> >0sec).
> >
> >My problem is that by turning the picture to Binary, some close vesicles
> >appear as one big object.
> >
> >How can i separate these vesicles? does anyone has a suggestion on a
> >different way of analysis?
> >
> >Thank you very much.
> >
> >Tal Shprung
> >
> >P.S.
> >
> >I want to thank the people behind this wonderful (and free) program. You
> >have made my research possible.
> >
>