http://imagej.273.s1.nabble.com/ImageJ-Vesicle-movement-analysis-tp3697136p3697139.html
channel that has the best contrast). In a grayscale image you
> Hi Martin,
>
> The watershed is not enough, it does seperate the objects but very
> slightly.
>
> I am looking for something that, still in the colored picture, will
> find the
> strongest points within each vesicle's center of mass and decress the
> vesicle's size a bit, to seperate the vesicles.
>
> On Feb 20, 2008 10:24 AM, M. Jaekel <
[hidden email]> wrote:
>
>> Hi Tal,
>>
>> you could maybe use the watershed algorithm to separate those close
>> objects. you can find the watershed function in one of the process
>> menu
>> options i think.
>>
>> best,
>> martin
>>
>> On Feb 20 2008, Tal Shprung wrote:
>>
>>> Dear all,
>>>
>>> I apologize if i repeat a previously asked question, but i was
>>> not sure
>> how
>>> to search for the solution.
>>>
>>> I load to the program two consecutive images (10sec apart) of a
>>> live cell
>>> with its vesicle fluorescently painted.
>>>
>>> what i get is two images with many vesicles (round shaped objects).
>>>
>>> what i need is to end up with some kind of number to describe the
>> movement
>>> of the vesicle (small movement/ short or long distance/
>>>
>>> a fraction of the vesicles moving)
>>>
>>> What i currently do is turn the pictures to Binary and adjust
>>> threshold
>> to
>>> auto and then subtract the second picture from the first.
>>>
>>> I measure the area resulted and divide that from the first
>>> picture (time
>>> 0sec).
>>>
>>> My problem is that by turning the picture to Binary, some close
>>> vesicles
>>> appear as one big object.
>>>
>>> How can i separate these vesicles? does anyone has a suggestion on a
>>> different way of analysis?
>>>
>>> Thank you very much.
>>>
>>> Tal Shprung
>>>
>>> P.S.
>>>
>>> I want to thank the people behind this wonderful (and free)
>>> program. You
>>> have made my research possible.
>>>
>>