http://imagej.273.s1.nabble.com/ImageJ-Vesicle-movement-analysis-tp3697136p3697140.html
> Hi Tal,
>
> you could try Process>Binary>Find Maxima with a noise tolerance
> that you have to find by trial and error (use preview).
>
> You can also convert the image to grayscale (or use one color
> channel that has the best contrast). In a grayscale image you
> can set a threshold to suppress background objects in
> Find Maxima.
>
> Michael
> ________________________________________________________________
>
> On 20 Feb 2008, at 13:08, Tal Shprung wrote:
>
> > Hi Martin,
> >
> > The watershed is not enough, it does seperate the objects but very
> > slightly.
> >
> > I am looking for something that, still in the colored picture, will
> > find the
> > strongest points within each vesicle's center of mass and decress the
> > vesicle's size a bit, to seperate the vesicles.
> >
> > On Feb 20, 2008 10:24 AM, M. Jaekel <
[hidden email]> wrote:
> >
> >> Hi Tal,
> >>
> >> you could maybe use the watershed algorithm to separate those close
> >> objects. you can find the watershed function in one of the process
> >> menu
> >> options i think.
> >>
> >> best,
> >> martin
> >>
> >> On Feb 20 2008, Tal Shprung wrote:
> >>
> >>> Dear all,
> >>>
> >>> I apologize if i repeat a previously asked question, but i was
> >>> not sure
> >> how
> >>> to search for the solution.
> >>>
> >>> I load to the program two consecutive images (10sec apart) of a
> >>> live cell
> >>> with its vesicle fluorescently painted.
> >>>
> >>> what i get is two images with many vesicles (round shaped objects).
> >>>
> >>> what i need is to end up with some kind of number to describe the
> >> movement
> >>> of the vesicle (small movement/ short or long distance/
> >>>
> >>> a fraction of the vesicles moving)
> >>>
> >>> What i currently do is turn the pictures to Binary and adjust
> >>> threshold
> >> to
> >>> auto and then subtract the second picture from the first.
> >>>
> >>> I measure the area resulted and divide that from the first
> >>> picture (time
> >>> 0sec).
> >>>
> >>> My problem is that by turning the picture to Binary, some close
> >>> vesicles
> >>> appear as one big object.
> >>>
> >>> How can i separate these vesicles? does anyone has a suggestion on a
> >>> different way of analysis?
> >>>
> >>> Thank you very much.
> >>>
> >>> Tal Shprung
> >>>
> >>> P.S.
> >>>
> >>> I want to thank the people behind this wonderful (and free)
> >>> program. You
> >>> have made my research possible.
> >>>
> >>
>