Re: Tracking movement along a line

Posted by Herbert M. Geller on Feb 12, 2008; 3:16pm
URL: http://imagej.273.s1.nabble.com/Tracking-movement-along-a-line-tp3697211p3697212.html

Why not make a kymograph?   This allows you to draw a line on the
kymograph by hand and then the slope of the line equals the velocity of
the movement.

The plug-in is here:
http://www.embl-heidelberg.de/eamnet/html/body_kymograph.html


Pedro J Camello wrote:

> I´m plannig to record transport of a rounded object along a line between
> the images of a stack. I don´t expect to have a clear-cut contrast between
> the object and the background. The aim is to get the speed (pixels/time)
> of the movement along the line
> I´ve thought of a handmade procedure:
> 1) draw a line
> 2) get line profile for each slice
> 3) put all the plot values in excel and calculate speed
>
> However, the number of images is too high to make this by hand. Thus, I
> need to automatize 2 and 3
>
> Any help will be wellcome
>
> Pedro
>
>
>  

--
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Herbert M. Geller, Ph.D.
Developmental Neurobiology Section
National Heart Lung and Blood Institute, NIH
10 Center Drive MSC 1754
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Bethesda, MD  20892-1754
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