area measurement of connected cells

Posted by Knecht, David on Feb 04, 2008; 6:26pm
URL: http://imagej.273.s1.nabble.com/area-measurement-of-connected-cells-tp3697279.html

I am trying to help a user calculate the area of cells in an image.  
The cells are all interconnected in a continuous sheet ( tissue  
section) with the membranes visible in the brightfield image.  The  
outlines can be fairly easily identified by thresholding, but I am not  
sure how to deal with the interconnected nature of the cells so that  
each closed unit is counted as a separate object.  The particle  
counter seems to want to count the whole area as one cell.  Thanks- Dave

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)