Setting scale of z step
Posted by Declan James on Jan 19, 2008; 9:07pm
URL: http://imagej.273.s1.nabble.com/Setting-scale-of-z-step-tp3697520.html
Dear ImageJ users
Could you lend me some help? I would like to set the z scale so that it
matches the z step size of the Nikon confocal stack. Each pixel has an
xyz of 0.4 X 0.4 X 0.1 um. At the moment after I set the scale within
Imagej I get pixels with xyz of 0.4 x 0.4 x 0.4 um(cubes). The stack is
imported into ImageJ using the very useful Ics opener. When I use the
ImageJ 3D viewer to create a projection I get an artifically elongated
image in the z axis.
Thanks
Declan