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Re: Logical operation on ROIs

Posted by Pang, Zhengyu (GE Global Research) on Jan 17, 2008; 6:08pm
URL: http://imagej.273.s1.nabble.com/Logical-operation-on-ROIs-tp3697543p3697545.html

Jerome,

Thanks for your reply. I found that your method is good if I manaully
trace the ROI1 and ROI2.

In my case, I specified my ROI based on its grayscale value (pixel value
> threshold) "Image>Adjust>Threshold" and then use the function
"Edit/Selection/Create Selection". I could not follow what you
instructed. Is there anyway to get around this?

Thanks,

Zhengyu

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Jerome Mutterer
Sent: Thursday, January 17, 2008 2:25 AM
To: [hidden email]
Subject: Re: Logical operation on ROIs

Zhengyu,

You can trace ROI1, then keep the shift key down and trace ROI2. Now
invert the selection using Edit/Selection/Make Inverse.
In a macro, use the setKeyDown('shift'); function.

Jerome


On Jan 17, 2008 4:00 AM, Pang, Zhengyu (GE, Research)
<[hidden email]>
wrote:

> Could I perform logical operation on ROIs? For example, If I have
> specified ROI1 and ROI2, could I define ROI3=whole image-ROI1-ROI2?
> And then perform necessary measurement on ROI3.
>
> Do I need to install some plugins to do it?
>
> Thanks!
>
> Zhengyu
> >               g Global Research Center
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> >           Zhengyu Pang, Ph.D.
>                        Bioanalytics and Protein Science Laboratory
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